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Extra resources for Applied Electrospray Mass Spectrometry (Practical Spectroscopy)
However, this is often impractical for time-varying experiments such as LC/MS. An alternative approach is to make use of a focal plane detector (348–350). It was already mentioned that decreasing the slit widths can result in a loss of sensitivity. Eliminating the collector slit to detect a wider range of mass-to-charge ratios simultaneously provides a multichannel advantage and improved sensitivity. Detection limits can be improved whenever the signal-to-noise ratio is limited by ion current and not chemical background.
Juraschek et al. (255) recently showed that nanospray can tolerate salt concentrations about an order of magnitude higher than can be handled with an IonSpray source. This is attributed to the formation of droplets about an order of magnitude smaller in nanospray than in conventional ESI sources and reduced competition between salt and analyte ions for the droplet surface as the ratio of surface area to droplet volume decreases. Nanospray methods are discussed in greater detail in Chapter 2. 2.
However, the combination of low cost, relatively high sensitivity, and MS/MS capabilities makes ion traps well suited for target compound screening and qualitative analysis of proteins and peptides. It should be noted that the quadrupole ion trap is not the only kind of ion trap. For example, Benner (420) reported on a unique gated electrostatic trap to measure the charge and mass-to-charge ratio of large ions generated by ESI. 2. Time-of-Flight Mass Spectrometers (433–441) Time-of-flight (TOF) mass spectrometers are experiencing a period of rapid growth.